Whole-brain long-range connectivity of glutamatergic, GABAergic, parvalbumin-expressing and somatostatin-expressing neurons in mouse somatosensory cortex

Understanding the composition of cortical circuits at the whole-brain scale is crucial. However, the specific ways in which particular neuronal types in the primary somatosensory cortex (SSp) establish connections with upstream and downstream brain regions remain unclear. In this study, we used whole-brain imaging technology with submicron resolution to systematically reveal the long-range connectivity patterns of glutamatergic, GABAergic, parvalbumin-expressing (PV+), and somatostatin-expressing (SOM+) neurons in the SSp. Our results show that while glutamatergic, GABAergic, PV+ , and SOM+ neurons receive similar upstream afferent, specific thalamic subregions showed numerically stronger afferent to GABAergic, PV+ , and SOM+ neurons compared to glutamatergic neurons. Additionally, glutamatergic neurons exhibit a more complex collateral projection pattern in subcortical axonal pathways compared to PV+ neurons. These findings elucidate the long-range connectivity patterns of specific neuronal types in the SSp, offering new insights into the cell-type-specific mechanisms of sensory information processing.