Accumulation of per- and polyfluoroalkyl substances (PFAS) in tissues of wild boar (Sus scrofa)

The widespread application of per- and polyfluoroalkyl substances (PFAS) resulted in ubiquitous environmental contamination. Understanding the PFAS tissue distribution in mammals and humans is crucial for the assessment of potential health risks. The levels of eleven PFAS were determined by UPLC-MS/MS in plasma and various tissues of wild boar (n = 82) hunted in Germany. The most prevalent PFAS (PFOA, PFNA, PFHxS and PFOS) were detected in all analyzed samples. The median level of the sum (Σ 4PFAS) was highest in liver (90.2 μg/kg), followed by kidney (9.45 μg/kg), plasma (7.63 μg/L), lung (6.84 μg/kg), heart muscle (2.60 μg/kg), spleen (2.46 μg/kg), and skeletal muscle (1.03 μg/kg). Consumption of a single portion (125 g) of liver containing the Σ 4PFAS median level would result in a 36.6-fold exceedance of the tolerable weekly intake (TWI) of EFSA in a 70 kg-person. The accumulation (calculated as tissue/plasma ratio) of perfluoroalkyl carboxylic acids with η_pfc = 8-13 in lung, spleen, muscle and heart tissues increased with molecule size, indicating passive mechanisms of distribution driven by hydrophobicity. In contrast, liver and kidney distribution coefficients scattered, indicating additional involvement of chain-length dependent active transport processes. The highest accumulation was observed for PFOS in the liver (median tissue/plasma ratio 18.0). The shortest PFAS included in the study (PFHxA, PFHpA, PFOA, PFBS and PFHxS) did not accumulate in any of the tissues, probably due to strong binding to blood proteins like serum albumin and their relative polarity impeding passive membrane diffusion.