Cost-Effective Motionless Color Coded Ptychography for High-Throughput Biomedical Imaging

High-throughput imaging methods are essential for various biomedical applications such as digital pathology, drug screening, and early stage cancer detection. Over the past few decades, numerous lensless techniques have emerged to provide high-throughput imaging with significantly more cost-effective hardware compared to conventional optical microscopy systems. Most lensless systems require sophisticated multiheight mechanical motions for phase retrieval, combined with subpixel shifts to reconstruct high-resolution images. These setups often involve bulky mechanical stages. Alternative approaches utilizing multiwavelength techniques can achieve similar physical shifts needed for phase retrieval but sacrifice color information in the process. We propose a novel coded ptychography lensless system capable of recovering high-resolution holographic images by using a single wavelength without any mechanical motion. Additionally, we leveraged an RGB LED matrix for color lensless imaging. Our compact lensless system demonstrates its capabilities by imaging various mouse tissues and organisms and oral cells offering a large field of view 30 mm2 with a resolution of 1.95 μm.