Full-Length Clonal Immunoglobulin Rearrangements in cfDNA: Improved Recovery and Sequencing

This is the first study to evaluate preanalytical and analytical factors that impact immunoglobulin sequencing in cfDNA. Our findings show optimal cfDNA isolation and library preparation procedures can improve the detection of clonal immunoglobulin rearrangements.

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Publication Details

Journal
Clinical Chemistry
Published
2025-05-02
DOI
https://doi.org/10.1093/clinchem/hvaf064
Primary Topic
Cancer Genomics and Diagnostics
Type
article
Field-Weighted Citation Impact
0.00

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article

Full-Length Clonal Immunoglobulin Rearrangements in cfDNA: Improved Recovery and Sequencing

Philippa Ashmore, Elizabeth Mayne, Christopher D. Gocke, Samantha L Vogt et al.
Clinical Chemistry
Cancer Genomics and Diagnostics
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Full-Length Clonal Immunoglobulin Rearrangements in cfDNA: Improved Recovery and Sequencing

Philippa Ashmore, Elizabeth Mayne, Christopher D. Gocke, Samantha L Vogt, Garrick Laudin, Prisca Mbonu, Tanvier Omar, Kevin He, Richard F. Ambinder, Moosa Patel, Lisa Haley, Sugeshnee Pather, Neil Alexander Martinson, Rena R. Xian, Emily R. Adams, Wendy Stevens, Ming-Tseh Lin, Atul Lakha, Vinitha Philip, Troy Billson
article en

Abstract

This is the first study to evaluate preanalytical and analytical factors that impact immunoglobulin sequencing in cfDNA. Our findings show optimal cfDNA isolation and library preparation procedures can improve the detection of clonal immunoglobulin rearrangements.

Clinical ChemistryVol. 71(8)
National Health Laboratory Service (ZA), Johns Hopkins University (US), University of Cape Town (ZA), University of the Witwatersrand (ZA), Johns Hopkins Medicine (US), Chris Hani Baragwanath Hospital (ZA), Netcare Unitas Hospital (ZA), Perinatal HIV Research Unit (ZA), Sidney Kimmel Comprehensive Cancer Center (US)
National Cancer Institute
Openalex Percentile: Top 84%
Cancer Genomics and Diagnostics
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