Full-Length Clonal Immunoglobulin Rearrangements in cfDNA: Improved Recovery and Sequencing
This is the first study to evaluate preanalytical and analytical factors that impact immunoglobulin sequencing in cfDNA. Our findings show optimal cfDNA isolation and library preparation procedures can improve the detection of clonal immunoglobulin rearrangements.
Authors
- Philippa Ashmore (ORCID: https://orcid.org/0000-0002-2654-3893)
- Elizabeth Mayne (ORCID: https://orcid.org/0000-0002-6360-3488)
- Christopher D. Gocke (ORCID: https://orcid.org/0000-0002-9541-2192)
- Samantha L Vogt
- Garrick Laudin (ORCID: https://orcid.org/0000-0002-6983-0438)
- Prisca Mbonu
- Tanvier Omar (ORCID: https://orcid.org/0000-0001-7636-7616)
- Kevin He (ORCID: https://orcid.org/0000-0002-8354-426X)
- Richard F. Ambinder (ORCID: https://orcid.org/0000-0003-4027-3073)
- Moosa Patel (ORCID: https://orcid.org/0000-0002-0647-4384)
- Lisa Haley (ORCID: https://orcid.org/0000-0002-8715-5667)
- Sugeshnee Pather (ORCID: https://orcid.org/0000-0003-1368-0028)
- Neil Alexander Martinson (ORCID: https://orcid.org/0000-0002-0184-5705)
- Rena R. Xian (ORCID: https://orcid.org/0000-0001-5532-6524)
- Emily R. Adams (ORCID: https://orcid.org/0000-0002-0816-2835)
- Wendy Stevens (ORCID: https://orcid.org/0009-0007-9393-5348)
- Ming-Tseh Lin
- Atul Lakha (ORCID: https://orcid.org/0009-0003-6680-1193)
- Vinitha Philip (ORCID: https://orcid.org/0009-0008-5503-9291)
- Troy Billson
Institutions
- National Health Laboratory Service (ZA)
- Johns Hopkins University (US)
- University of Cape Town (ZA)
- University of the Witwatersrand (ZA)
- Johns Hopkins Medicine (US)
- Chris Hani Baragwanath Hospital (ZA)
- Netcare Unitas Hospital (ZA)
- Perinatal HIV Research Unit (ZA)
- Sidney Kimmel Comprehensive Cancer Center (US)
Publication Details
- Journal
- Clinical Chemistry
- Published
- 2025-05-02
- DOI
- https://doi.org/10.1093/clinchem/hvaf064
- Primary Topic
- Cancer Genomics and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Cancer Institute