Construction of stable packaging cell lines for large-scale industrial BaEV-enveloped retroviral vector production
Compared with the existing methods, the transduction efficiency of the retroviral vector produced by BaEV-PackRV was significantly greater in primary immune cells at a lower multiplicity of infection (MOI), and the transduced CAR-T or CAR-NK cells maintained good expansion capacity and enhanced cytotoxicity. On this basis, our system enables large-scale industrial production of BaEV-coated retroviral vectors while significantly reducing costs.This will greatly improve the efficacy of NK cell gene transfer and the effectiveness of related treatments.
Authors
- Xiaorui Li (ORCID: https://orcid.org/0009-0007-5268-0166)
- Sihui Zhan (ORCID: https://orcid.org/0000-0002-1575-5946)
- Zhuoying Yu
- Jin‐Fu Xu (ORCID: https://orcid.org/0000-0002-8039-8973)
- Yaru Feng (ORCID: https://orcid.org/0000-0002-3459-8368)
- Yuanyuan Shi (ORCID: https://orcid.org/0000-0003-2997-4573)
- Chunting Qiu
- Jianxun Wang (ORCID: https://orcid.org/0000-0002-3731-6514)
- Hanyi Chen (ORCID: https://orcid.org/0000-0002-0300-3720)
- Weijia Wang (ORCID: https://orcid.org/0000-0003-0164-9322)
- Ping Ma (ORCID: https://orcid.org/0000-0002-0585-7885)
- Lijun Zhao (ORCID: https://orcid.org/0000-0002-0193-3860)
- Jiaqi Fan (ORCID: https://orcid.org/0000-0002-2769-1185)
- Qihong Ma
Institutions
- Beijing University of Chinese Medicine (CN)
- Fudan University (CN)
- Sino Biopharmaceutical (China) (CN)
- Huadong Hospital (CN)
- Tianjin Infectious Diseases Hospital (CN)
- Zhongshan People's Hospital (CN)
- Tianjin People's Hospital (CN)
- Beike Biotechnology (China) (CN)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-05-26
- DOI
- https://doi.org/10.3389/fimmu.2025.1578660
- Citations
- 1
- Primary Topic
- Viral Infectious Diseases and Gene Expression in Insects
- Type
- article
- Field-Weighted Citation Impact
- 0.81