Development of an Endo-glycoside Hydrolase Assay System Using Synthetic Glycan Probes

Endo-β-N-acetylglucosaminidases (ENGases) are endoglycosidases that hydrolyze asparagine-linked glycan on glycoproteins. These enzymes play a crucial role in the structural analysis of glycoprotein glycans due to their hydrolytic activity and facilitate the synthesis of glycoproteins with homogeneous glycan structures through glycosylation activity. In this study, we present an approach to detect the hydrolysis and transglycosylation activities of ENGase using glycan-based molecular probes.