Establishment of a CRISPR-Cas9-mediated keap1aknockout protocol in zebrafish (Danio rerio) for implementation in Vietnam

This pioneering study inVietnam employed clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9 (CRISPR-Cas9) technology to generate Kelch-like ECH-associated protein 1a (keap1a) knockout zebrafish (Danio rerio), aiming to investigate the Keap1-Nuclear factor erythroid 2-related factor 2 (Keap1-Nrf2) system's role in cellular oxidative stress protection.Zebrafish, possessing two keap1 paralogs (keap1a and keap1b), serve as an ideal model organism for this investigation.The experimental procedure involved designing a specific guide RNA (gRNA) targeting the keap1a gene and microinjecting the gRNA/Cas9 complex into single-cell stage zebrafish embryos.While keap1a knockout fish exhibited normal development without apparent phenotypic abnormalities or