Antimicrobial evaluation of bullfrog skin for the development of dressing for infected wounds

Studies have been conducted to develop dressings that reduce the effects of contamination in infected wounds and promote the healing process at a low cost. In this context, there is an urgent need to improve and create new inputs for producing effective dressing materials capable of combating infectious agents in wounds. The present study aimed to evaluate the bacteriostatic and/or bactericidal action of bullfrog skin (Aquarana catesbeiana), a byproduct of the frog farming industry, to reduce hospital costs using dressing beds made with this biomaterial. The tests were conducted in vitro against two strains of Staphylococcus aureus, with the goal of reducing dressing costs using a bed made from this sterilized natural biomaterial. The processing, decontamination, and sterilization of the bullfrog skin were carried out by autoclaving, this methodology was based on the recommendations of Santos et al. (2020). Antibiogram tests were performed using disk diffusion and compared to commercial antibiotics. The results of the microbiological analyses on the pulverized and autoclaved bullfrog skin indicated that this sterilization method was effective, contributing to lower production costs for the new technological process. Antimicrobial activity was observed against both strains of Staphylococcus aureus, suggesting that bullfrog skin has potential for use as a wound dressing bed.