PHENOLIC CONSTITUENTS OF TWIGS AND LEAVES OF WIKSTROEMIA INDICA (L.) C.A. MEY. AND THEIR EFFECTS ON LPS-INDUCED IL-1β AND IL-10 CYTOKINE PRODUCTION IN RAW 264.7 CELL LINES

Five phenolic compounds, including methyl 4-hydroxybenzoate (1), syringin (2), genkwanin (3), kaemferol-3-O-rhamnoside (4), and yuankanin (5), were isolated from the 70% EtOH extract of W. indica twigs and leaves. Their structures were identified using nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS) datawith compound 2 being reported for the first time from this plant. The compounds were evaluated for their effects on IL-1β and IL-10 cytokine production in LPS-induced RAW 264.7 macrophages and 3 showed considerably inhibitory activity against IL-1β with an IC50 value of 18.8  2.46 µM.