In-vitro Anticancer Activity of Ethanol and Aqueous Extract of Leaves and Stem of Cissus woodrowii (Stapf Ex T. Cooke) Santapau against HepG2 Liver Cancer Cell Line

Background and purpose: Researchers looked at the HepG2 liver cancer cell line to see whether ethanol and an aqueous extract of Cissuswoodrowii leaves and stems had any anticancer effects. Experimental approach: Using the soxhlet equipment, ethanol and water-based plant extracts were produced for this study. Using the MTT assay, trypan blue, and brine shrimp lethality assay, in-vitro anticancer action of certain plant extracts was investigated at doses ranged from 10 to 100µg/ml against designated cell line. Findings/Results: The anticancer action was achieved with the usage of 5-flurouracil. In comparison to the water-based stem and leaf extracts of CissuswoodrowiiSantapau, ethanolic stem extract had the greatest % of inhibition against the HepG2 cell line, along with effective IC50 values. Conclusion and implication: In conclusion, our results suggest that the 50% ethanol-aqueous stem and leaves extract of Cissuswoodrowii (Stapf Ex T. Cooke) Santapu showed a clear cytotoxic and brine shrimp lethality inductive impact on the human hepatoma (HepG2) cell line, suggesting an anticancer activity.