Development and Validation of a Bioanalytical LC-MS/MS Method for Detection of Dasatinib in Rat Plasma

This work presents a novel method for detection of concentration of Dasatinib in rat plasma using Verapamil as internal standard by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method's pharmacokinetic application in rats was also evaluated. Separation was achieved using AB Sciex 4000 instrument, equipped with a Chromasol Garnet C18 polar analytical column (50 × 4.6 mm, 4 μm, 120 Å). The column oven was maintained at 45 ± 2°C, while the autosampler was kept at 10 ± 2°C. The mobile phase consisted of a 60:40 v/v mixture of water and methanol, delivered at flow rate of 0.8 mL/min, with 2 μL an injection volume. The liquid chromatographic procedure was carried out over a four-minute duration. The mass spectrometer operated in positive electrospray ionization molecular structure (+ESI) mode, employing multiple reaction monitoring (MRM) to detect the mass-to-charge ratio transitions for Dasatinib (m/z 488.1 → 401.0) and Verapamil (internal standard, m/z 455.6 → 165.3). were determined using multiple reaction monitoring (MRM). The concentration range for Dasatinib was 500 to 1 ng/mL, with a correlation coefficient of 0.9996. The accuracy and precision for MQC, HQC, LLQC, and LQC were 106.09 %, 107.33%, 99.83%, and 94.15%, respectively. The developed LC-MS/MS method is simple, precise, sensitive and reproduceable. Henceforth it can be used to determine dasatinib.