Phytochemical Profiling and Gas Chromatography-Mass Spectrometry Analysis of Bioactive Compounds in Methanol Extract of Indigofera prostrata

Background: The contemporary study emphases on identification of bioactive components from methanol extract of Indigofera prostrata by Gas chromatography-mass spectrometry investigation and similarly evaluation of phytochemical components that are existing in Indigofera prostrata in methanol extract is also done. Objective: The objective was to accomplish Standardisation, to distinguish, authenticate as well as to prevent the adulteration of Indigofera prostrata. Materials and methods: Standardisation, Preliminary phytochemical screening and GC-MS study was accomplished. A mass spectrophotometer and 7890A gas chromatograph system (GCMSQP2010, SHIMADZU) was utilized to perform the GC-MS analysis. Analysis of Gas chromatography mass spectrometry with methanol extract of Indigofera prostrata had revealed the occurrence of diverse kinds of bio active constituents. Results: Phytochemical screening of the extract of methanol of Indigofera prostrata revealed the existence of tannins, flavonoids, compounds of phenol, carbohydrates, amino acids, saponins, triterpenoids, proteins and steroids. Totally 14 compounds had been identified in methanol extract composition of Indigofera prostrata & had revealed bio active 1- Butanol, 3-methylformate, β-Acorenol, 9,12-Octadecadienoicacid, Hexadecanoic acid, and d-Mannose, methyl ester, 3-O- Methyl-d-glucose, phytol, Tetradecen-1-olacetate (E-8-Methyl-9), Other ingredients include 2-hydroxy-1- (hydroxymethyl) ethyl ester Campesterol, γ-Sitosterol, Stigmasterol, 2-hydroxy-1(hydroxymethyl) ethyl ester, and luteol. The findings of the experiments mentioned above are as follows: the figures for total ash and loss on drying are 8.35 ± 0.35 and 7.45 ± 0.02 correspondingly, water soluble ash was 3.65 ± 0.04. Conclusion: These compounds are said to be responsible for the different pharmacological and therapeutic properties. The results from the current study provides insightful information that will help select and carry out future research projects.