hiPSC-derived cardiac fibroblasts dynamically enhance the mechanical function of hiPSC-derived cardiomyocytes on an engineered substrate
These data suggest that hiPSC-CFs exert dynamic and multifactorial effects on the mechanical function of hiPSC-CMs and highlight the importance of CFs in both the native heart and <i>in vitro</i> cardiac models. Further, this work demonstrates the applicability of the coculture-conditioned medium-monoculture paradigm to decouple the effects of paracrine factor and cell-cell signaling on hiPSC-CM mechanical function and maturation.
Authors
- Timothy J. Kamp (ORCID: https://orcid.org/0000-0003-2103-7876)
- Corey L. Anderson (ORCID: https://orcid.org/0000-0002-6898-8479)
- Lee L. Eckhardt (ORCID: https://orcid.org/0000-0001-5792-064X)
- Alana Stempien (ORCID: https://orcid.org/0000-0002-6716-5876)
- Wendy C. Crone (ORCID: https://orcid.org/0000-0002-6956-4221)
- Mitchell Josvai (ORCID: https://orcid.org/0009-0008-0574-6991)
- Meghana Kalluri (ORCID: https://orcid.org/0009-0008-2185-4511)
- Jianhua Zhang (ORCID: https://orcid.org/0000-0002-3819-2437)
- Jodi Lawson
- Harshal Kanade
Institutions
- University of Wisconsin–Madison (US)
- Wisconsin Institutes for Discovery (US)
Publication Details
- Journal
- Frontiers in Bioengineering and Biotechnology
- Published
- 2025-05-23
- DOI
- https://doi.org/10.3389/fbioe.2025.1546483
- Citations
- 5
- Primary Topic
- Tissue Engineering and Regenerative Medicine
- Type
- article
- Field-Weighted Citation Impact
- 10.03
Funders
- National Science Foundation
- University of Wisconsin-Madison
- National Institutes of Health