3D Temperature Mapping During Photothermal Therapy in Cellular Assemblies

Control over temperature in three space dimensions and time is of utmost importance in many contexts, including photothermal therapies, where it is expected to provide valuable insights and enhance clinical outcomes. Current techniques for noncontact temperature mapping during hyperthermia are restricted to 2D imaging, due to the lack of strategies able to provide images in suitable time frames for the inevitable large amount of information encompassed in 3D tomography. Iron selenide nanoparticles (NPs) are developed with high temperature sensitivity that, in combination with low‐field magnetic resonance imaging, enable 3D temperature imaging in the ≈20 s time frame. This approach is suitable for monitoring and evaluating photothermal therapy applied to cellular assemblies, which typically occurs over a time frame of tens of minutes. In addition, iron selenide NPs behave, simultaneously, as photothermal and thermometric agents embedded in cellular models at concentrations where their cytotoxicity is low. This dual functionality enables to control the depth achieved by the therapy with a temperature accuracy ≤1 °C, enabling the detection and control of hot spots that would be otherwise overlooked. Overall, this is a universal approach for temperature mapping in 3D and for localized heating in virtually any living tissue.

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Publication Details

Journal
Small Structures
Published
2025-05-23
DOI
https://doi.org/10.1002/sstr.202400658
Primary Topic
Photoacoustic and Ultrasonic Imaging
Type
article
Field-Weighted Citation Impact
0.00

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article

3D Temperature Mapping During Photothermal Therapy in Cellular Assemblies

Ángel Millán, Manuel Mariani, Vítor M. Gaspar, Claudia Innocenti et al.
Small Structures
Photoacoustic and Ultrasonic Imaging
article

3D Temperature Mapping During Photothermal Therapy in Cellular Assemblies

Ángel Millán, Manuel Mariani, Vítor M. Gaspar, Claudia Innocenti, João F. Mano, Filipa L. Sousa, Rui Oliveira‐Silva, Nuno J. O. Silva, A. Lascialfari, Joana F. Soeiro, Rute A. Pereira
article en

Abstract

Control over temperature in three space dimensions and time is of utmost importance in many contexts, including photothermal therapies, where it is expected to provide valuable insights and enhance clinical outcomes. Current techniques for noncontact temperature mapping during hyperthermia are restricted to 2D imaging, due to the lack of strategies able to provide images in suitable time frames for the inevitable large amount of information encompassed in 3D tomography. Iron selenide nanoparticles (NPs) are developed with high temperature sensitivity that, in combination with low‐field magnetic resonance imaging, enable 3D temperature imaging in the ≈20 s time frame. This approach is suitable for monitoring and evaluating photothermal therapy applied to cellular assemblies, which typically occurs over a time frame of tens of minutes. In addition, iron selenide NPs behave, simultaneously, as photothermal and thermometric agents embedded in cellular models at concentrations where their cytotoxicity is low. This dual functionality enables to control the depth achieved by the therapy with a temperature accuracy ≤1 °C, enabling the detection and control of hot spots that would be otherwise overlooked. Overall, this is a universal approach for temperature mapping in 3D and for localized heating in virtually any living tissue.

Small StructuresVol. 6(6)
Istituto Nazionale di Fisica Nucleare (IT), University of Pavia (IT), Universidad de Zaragoza (ES), Istituto Nazionale di Fisica Nucleare, Sezione di Pavia (IT), Institute for the Chemistry of OrganoMetallic Compounds (IT), Instituto de Nanociencia y Materiales de Aragón (ES), National Interuniversity Consortium of Materials Science and Technology (IT), University of Florence (IT), University of Aveiro (PT)
European Commission, Ministerio de Economía y Competitividad, Ministério da Ciência, Tecnologia e Ensino Superior, Istituto Nazionale di Fisica Nucleare, Centro de Investigação em Materiais Cerâmicos e Compósitos, Fundação para a Ciência e a Tecnologia, Instituto Nazionale di Fisica Nucleare
Openalex Percentile: Top 89%
Photoacoustic and Ultrasonic Imaging
DOIOpen Access PDFOpenAlex
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