A 4-Methylbenzoylhydrazine Pt(II) Complex Inhibits the Proliferation of Breast Cancer Cells by Regulating the Cell Cycle and Inducing Apoptosis

In this study, a novel 4-methylbenzoylhydrazide·dimethyl sulfoxide·dichloro platinum(II) complex (Pt2) was synthesized and characterized, and its anti-tumor activity and action mechanism were explored. The molecular structure and spatial configuration of the complex were determined using X-ray diffraction. The results obtained using fluorescence spectroscopy demonstrate that this complex can effectively bind to DNA and affect its fluorescence properties. The experimental results show that Pt2 exhibited significant inhibitory effects on a variety of tumor cell lines (MCF-7, HepG-2, NCI-H460, T24, and A549), and its IC50 values were lower than those of cisplatin (DDP), indicating stronger anti-tumor activity. In addition, the complex not only significantly induced the apoptosis of MCF-7 cells but also inhibited cell cycle arrest at the G2 phase, with the proportion of G2-phase cells as high as 49.47%. In conclusion, the 4-methylbenzoylhydrazide platinum(II) complex exhibits good anti-tumor activity by inducing apoptosis and inhibiting the cell cycle, providing an important experimental basis for the development of novel platinum-based anti-tumor drugs.