Crystal structure of the CCA-adding enzyme from Arabidopsis thaliana

The 3'-terminal CCA-end of tRNA is essential for the attachment of amino acids and correct positioning of the aminoacyl-tRNA in the ribosome. In higher plants, the CCA sequence is synthesized, maintained, and repaired by class-II CCA-adding enzymes encoded by a single nuclear gene but multi-targeted to the nucleus, cytoplasm, plastids, and mitochondria. The structure of plant class-II CCA-adding enzyme remains unsolved. Here we describe the crystal structure of CCA-adding enzyme from <i>Arabidopsis thaliana</i> (<i>At</i>CCA)<i>.</i> The overall structure of <i>At</i>CCA is similar to other class-II CCA-adding enzymes<i>,</i> but significant differences occur in the body domain. Structural comparison of body and tail domains between <i>At</i>CCA and other class-II CCA-adding enzymes unravels three specific regions of <i>At</i>CCA. Based on the modeled <i>At</i>CCA-tRNA complex, <i>At</i>CCA may have a different tRNA binding pattern. The three specific regions located in the body domain of <i>At</i>CCA also provide candidate regions for multi-targeted sorting.