Enhanced neurotropism of bovine H5N1 compared to the Vietnam H5N1 isolate in C57BL/6J mice
In this study, we investigated differences in tissue tropism of two HPAI H5N1 strains, the isolate A/Vietnam/1203/2004 (VN1203) isolated from a fatal human case in 2004 and the bovine isolate A/Bovine/Ohio/B24osu-342/2024 (Bov342) isolated in 2024, in C57BL/6J mice. Infection via aerosols was uniformly lethal in mice. However, tissue tropism differed significantly: while VN1203 replication was largely restricted to the respiratory tract, Bov342 successfully replicated in the respiratory tract as well as various regions of the brain. Correspondingly, cytokine profiles in the brain differed significantly between the isolates. Notably, in addition to abundant evidence of CNS infection in Bov342-challenged mice via immunohistochemistry, sporadic intranuclear and intracytoplasmic immunoreactivity was observed in other tissues in the head, including the choroid plexus, retina, and inner ear. This study demonstrates that while both HPAI H5N1 isolates are uniformly lethal in C57BL/6J mice upon aerosol exposure, significant differences exist in tissue tropism.
Authors
- Claude Kwe Yinda (ORCID: https://orcid.org/0000-0002-5195-5478)
- Kerry Goldin (ORCID: https://orcid.org/0000-0001-5448-4088)
- Neeltje van Doremalen (ORCID: https://orcid.org/0000-0003-4368-6359)
- Vincent J. Munster (ORCID: https://orcid.org/0000-0002-2288-3196)
- Reshma Koolaparambil Mukesh (ORCID: https://orcid.org/0000-0002-2046-2793)
- Greg Saturday (ORCID: https://orcid.org/0000-0002-0803-6177)
- Jonathan E. Schulz (ORCID: https://orcid.org/0000-0001-5462-5952)
- Craig Martens (ORCID: https://orcid.org/0000-0001-8112-2234)
- Randall C. Johnson (ORCID: https://orcid.org/0000-0001-7754-0847)
- Sarah van Tol (ORCID: https://orcid.org/0000-0002-9905-920X)
- Kendal G. Cooper (ORCID: https://orcid.org/0000-0003-2699-2610)
- Emmie de Wit (ORCID: https://orcid.org/0000-0002-9763-7758)
- Jessica Prado-Smith (ORCID: https://orcid.org/0009-0009-3367-8348)
- Shane Gallogly (ORCID: https://orcid.org/0000-0001-6161-9104)
Institutions
- National Institutes of Health (US)
- National Institute of Allergy and Infectious Diseases (US)
Publication Details
- Journal
- npj Viruses
- Published
- 2025-05-23
- DOI
- https://doi.org/10.1038/s44298-025-00121-0
- Citations
- 5
- Primary Topic
- Influenza Virus Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 6.77
Funders
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases