Cu‐O ₄ Single Atom Carbon Dots Nanozymes for Efficient Self‐Adaptive Bacteria‐Infected Wound Therapy

Abstract Developing single‐atom nanozymes with optimized ligand structures and multienzyme activities for self‐adaptive infected wound therapy presents a major challenge. Herein, single‐atom carbon dots with CuO 4 coordination environment (Cu‐SLCDs) and CuN 4 coordination environment (CuN‐CDs) are prepared, respectively. Both materials exhibit multienzyme‐like (oxidase‐, peroxidase‐, superoxide dismutase‐, catalase‐like) activities, with Cu‐SLCDs showing higher activity levels. Experiments and theoretical calculations demonstrate that, compared to CuN‐CDs, the polyphenol p‐π conjugated structure creates a unique electron‐donating coordination environment, which increases the electron density of Cu‐SLCDs at the Fermi level, enhances electron transfer, and boosts multienzyme‐like activities. In vitro and in vivo experiments demonstrated that Cu‐SLCDs effectively eliminate over 98.6% of bacteria at a concentration of 25 µg mL −1 , while reducing reactive oxygen species levels in normal cells to promoting efficient wound healing. The development of Cu‐SLCDs with optimized CuO 4 coordination environment provides a novel approach for infected wound therapy and broadens the potential applications of single‐atom nanozymes.