Enzyme histochemistry of uterine tube of buffalo during follicular and luteal phase: A seasonal variation

Enzyme histochemistry on uterine tube of buffalo during summer and winter season was conducted in different phases of estrus cycle in relation to reproductive efficiency. Fresh tissues of buffalo uterine tubes were collected from infundibulum, ampulla and isthmus immediately after slaughtering. Cryostat sections were obtained and incubated to find variation in localization of various enzymes. AKPase showed strong to intense activity in winter and strong during summer season in the lamina epithelialis whereas negligible to weak reaction was found in lamina propria, propria submucosa, tunica muscularis and tunica serosa of uterine tube during both the seasons. Succinic dehydrogenase showed strong reaction in winter and moderate to strong during summer in the lamina epithelialis of uterine tube whereas reaction was moderate to strong in tunica muscularis, weak in the tunica serosa and negligible to weak in the lamina propria of uterine tube during both the seasons. G-6-P-D showed strong to intense reaction during winter and summer in the lamina epithelialis of uterine tube whereas reaction was negligible to weak in lamina propria, weak in propria submucosa, moderate to strong in the tunica muscularis and negligible to weak in the tunica serosa of uterine tube. NADH-diaphorase and NADPH- diaphorases showed intense reaction during both summer and winter in lamina epithelialis, moderate to strong reaction in tunica muscularis, weak to moderate reaction in lamina propria and propria submucosa, and weak reaction in tunica serosa of infundibulum, ampulla and isthmus during both the seasons. The activity of the enzymes was found to be more in winter and in luteal phase as compared to summer and follicular phase.