PCR based identification of different strongyle species in goats of western Uttar Pradesh, India

Strongyle infection in goats poses a significant threat to the agricultural industry leading to substantial economic losses through decreased productivity and increased mortality rates. The current study’s goal is to determine the molecular- based methods for identification of different kind of strongyle eggs from naturally infected goats from four selected districts of Western Uttar Pradesh. A total of 360 faecal samples were collected, with 32.22% testing positive for strongyle infection. The positive faecal samples were pooled separately and subjected to larval culture, followed by DNA extraction from the L3 larvae. Polymerase chain reaction (PCR) analysis of the ITS-2 region of the rDNA confirmed the presence of Haemonchus contortus, Trichostrongylus and Oesophagostomum genera. The prevalence rates of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. were determined to be 54.16%, 31.66% and 14.16% respectively. The finding of this study highlighted Haemonchus contortus as the predominant species among the identified strongyle spp. These findings provide valuable insights into the distribution of different types of strongyle infection in goats, which can help in management of parasites and their control in goats.