Linking Epitope‐Specific T‐Cell Receptors to IFNγ Secretion Using Nanovial Technology
To link IFNγ-secretion levels of epitope-specific T-cells with their TCRαβ, we coated nanovials with pHLA-I to capture and activate epitope-specific T-cells and their secreted IFNγ, followed by index-sorting and TCRαβ sequencing. We demonstrate that nanovials are a promising tool to link single epitope-specific TCRαβ clonotypes to the cell's functional properties.
Authors
- Tejas Menon (ORCID: https://orcid.org/0000-0001-7636-0051)
- Jet van den Dijssel (ORCID: https://orcid.org/0000-0002-4849-0374)
- Klaas P. J. M. van Gisbergen (ORCID: https://orcid.org/0000-0003-3623-7555)
- Hayley A. McQuilten (ORCID: https://orcid.org/0000-0002-7089-5300)
- Alexis González (ORCID: https://orcid.org/0000-0003-3741-6854)
- Sevana Baghdasarian
- Carolien E. van de Sandt (ORCID: https://orcid.org/0000-0002-4155-7433)
- Anja ten Brinke (ORCID: https://orcid.org/0000-0002-0135-7496)
- Citradewi Soemardy
- Oliver Eltherington
- Dino Di Carlo (ORCID: https://orcid.org/0000-0003-3942-4284)
Institutions
- Champalimaud Foundation (PT)
- California NanoSystems Institute (US)
- University of California, Los Angeles (US)
- The University of Melbourne (AU)
- Sanquin (NL)
- Peter Doherty Institute (AU)
- Fluxion Biosciences (United States) (US)
- Amsterdam University Medical Centers (NL)
- University of Amsterdam (NL)
Publication Details
- Journal
- European Journal of Immunology
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1002/eji.202451666
- Citations
- 1
- Primary Topic
- CAR-T cell therapy research
- Type
- article
- Field-Weighted Citation Impact
- 1.05
Funders
- Silicon Valley Community Foundation
- University of Melbourne
- Stichting Sanquin Bloedvoorziening
- Australian Research Council