HPTLC and U.V. Spectrophotometer as Tools for the Determination of Curcumin and Piperine in Zandu Haldi drops: A standardization approach

Zandu Haldi Drops are a one-of-a-kind recipe intended to promote immunity and overall health. It possesses the goodness of saffron, cinnamon, cardamom, black pepper, and Haldi. The present work was designed to estimate Curcumin (Turmeric) by High-Performance Thin Layer Chromatography (HPTLC) as well as Piperine (Pepper) through UV Spectrophotometry, with reference to the marker compounds. A standard curve was prepared separately for Curcumin and Piperine using methanol as solvent. HPTLC plate was scanned in scanner at 421 nm. Sample peak area values were compared with that of standard's area to determine the extent of drug present. The standard plot for Piperine was prepared by scanning the known concentrations at 342 nm and the unknown sample concentration was determined from this curve. The concentration of Curcumin was found to be 5.6 μg/ml and the Piperine concentration in the formulation was 2.5%. From the obtained results, it can be concluded that the formulation of Zandu haldi drops confirmed the presence of labelled ingredients viz, Turmeric and Pepper.