Finding an informative multilocus polymorphism in sunflower broomrape populations

Multilocus polymorphic DNA analysis was performed using 13 ISSR markers for 14 broomrape populations of Serbian and Moldovan origin. 279 ISSR fragments were identified for the whole sample, of which 265 (94.98%) were polymorphic. The seven groups of broomrape studied (whole sample, by country separately - Serbia and Moldova, and by race depending on the country - races ≤E serb, ≤E mold, H mold, H+ mold) showed different levels of genetic diversity. Based on these studies’ results, broomrape’s multilocus polymorphism was highly correlated with geographical origin, racial classification, and genetic background.