Development and Concordance of Binding and Neutralizing Assays to Determine SARS-CoV-2 Antibody Activity in Human Milk
SARS-CoV-2 binding and neutralizing antibody activity in HBM was determined using standardized and rigorous assays. HBM positivity cutoff values for SARS-CoV-2 vaccination and infection were established. The methods and approach described here could be applied to other pathogens and mucosal secretions.
Authors
- Kelly E. Manning (ORCID: https://orcid.org/0000-0002-6262-1190)
- Kathryn M. Moore (ORCID: https://orcid.org/0000-0002-8483-1148)
- Bushra Wali (ORCID: https://orcid.org/0000-0001-9278-8744)
- Navdeep Grewal
- Cristina V. Cardemil (ORCID: https://orcid.org/0000-0002-5898-6154)
- Patricia L. Milletich (ORCID: https://orcid.org/0000-0002-7660-3057)
- Zhiyi Zhu (ORCID: https://orcid.org/0009-0001-9361-7856)
- Mehul S. Suthar (ORCID: https://orcid.org/0000-0002-2686-8380)
- Sasha E. Larsen (ORCID: https://orcid.org/0000-0001-5034-9727)
- Madison Ellis (ORCID: https://orcid.org/0000-0002-0325-5477)
- Kathleen M. Neuzil (ORCID: https://orcid.org/0000-0001-9480-2714)
- Bryan J. Berube (ORCID: https://orcid.org/0000-0002-3806-040X)
- Mallory Shriver (ORCID: https://orcid.org/0009-0006-6536-3218)
- Rhea N. Coler (ORCID: https://orcid.org/0000-0001-6974-5666)
- Marcela F. Pasetti (ORCID: https://orcid.org/0000-0003-0894-4009)
- Alberto Moreno (ORCID: https://orcid.org/0000-0002-3031-7194)
- Flor M. Muñoz
- Christine M. Posavad
- Ines A Cadena
Institutions
- University of Maryland, Baltimore (US)
- Center for Infectious Disease Research (US)
- National Institutes of Health (US)
- Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa (ZA)
- Infectious Disease Research Institute (US)
- Emory University (US)
- Emory and Henry College (US)
- University of Washington (US)
- Fred Hutch Cancer Center (US)
- National Institute of Allergy and Infectious Diseases (US)
- Children's Healthcare of Atlanta (US)
- Texas Children's Hospital (US)
- Emory National Primate Research Center
Publication Details
- Journal
- Pathogens and Immunity
- Published
- 2025-05-22
- DOI
- https://doi.org/10.20411/pai.v10i2.799
- Primary Topic
- SARS-CoV-2 and COVID-19 Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00