Development and Concordance of Binding and Neutralizing Assays to Determine SARS-CoV-2 Antibody Activity in Human Milk

SARS-CoV-2 binding and neutralizing antibody activity in HBM was determined using standardized and rigorous assays. HBM positivity cutoff values for SARS-CoV-2 vaccination and infection were established. The methods and approach described here could be applied to other pathogens and mucosal secretions.

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Publication Details

Journal
Pathogens and Immunity
Published
2025-05-22
DOI
https://doi.org/10.20411/pai.v10i2.799
Primary Topic
SARS-CoV-2 and COVID-19 Research
Type
article
Field-Weighted Citation Impact
0.00
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article

Development and Concordance of Binding and Neutralizing Assays to Determine SARS-CoV-2 Antibody Activity in Human Milk

Kelly E. Manning, Kathryn M. Moore, Bushra Wali, Navdeep Grewal et al.
Pathogens and Immunity
SARS-CoV-2 and COVID-19 Research
article

Development and Concordance of Binding and Neutralizing Assays to Determine SARS-CoV-2 Antibody Activity in Human Milk

Kelly E. Manning, Kathryn M. Moore, Bushra Wali, Navdeep Grewal, Cristina V. Cardemil, Patricia L. Milletich, Zhiyi Zhu, Mehul S. Suthar, Sasha E. Larsen, Madison Ellis, Kathleen M. Neuzil, Bryan J. Berube, Mallory Shriver, Rhea N. Coler, Marcela F. Pasetti, Alberto Moreno, Flor M. Muñoz, Christine M. Posavad, Ines A Cadena
article en

Abstract

SARS-CoV-2 binding and neutralizing antibody activity in HBM was determined using standardized and rigorous assays. HBM positivity cutoff values for SARS-CoV-2 vaccination and infection were established. The methods and approach described here could be applied to other pathogens and mucosal secretions.

Pathogens and ImmunityVol. 10(2)
University of Maryland, Baltimore (US), Center for Infectious Disease Research (US), National Institutes of Health (US), Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa (ZA), Infectious Disease Research Institute (US), Emory University (US), Emory and Henry College (US), University of Washington (US), Fred Hutch Cancer Center (US), National Institute of Allergy and Infectious Diseases (US), Children's Healthcare of Atlanta (US), Texas Children's Hospital (US), Emory National Primate Research Center
Openalex Percentile: Top 85%
SARS-CoV-2 and COVID-19 Research
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