Changes in extracellular collagen and caspase-3 expression in testis of male mice due to frequent exposure to magnetic resonance imaging (MRI); Histochemical and immunohistochemical studies

Magnetic fields included in magnetic resonance imaging (MRI) technology are now utilized extensively in diagnostic medicine and healthcare applications.Simultaneously, it provides potential risks to human health, and their biological impacts on the human body are garnering heightened inquiry.The primary objective of the current study was to examine the biological effects of a high-strength magnetic field produced by 1.5 Tesla static magnetic fields on male mice spermatogenesis parameters in magnetic resonance imaging. Material and methods:In this study, 20 adult male mice were exposed to a 1.5 T MRI field for four weeks (two sessions per week, 1 hour each).Animals were sacrificed at the end of 4th week.Histopathological, histochemical, and Caspase-3 immunohistochemical examinations were performed.Results: Exposure to MRI fields in male mice led to structural and functional damage in the testes, including degeneration of germ and Leydig cells, heightened testicular cell apoptosis, elevated collagen deposition, and upregulated caspase-3 expression. Conclusion:Exposure to MRI magnetic fields led to notable histological, histochemical, and immunohistochemical alterations in the mice testes at the conditions of the current experiment.