A urine survivin (BIRC5) bioluminescence-based immunoassay
A highly sensitive solid-phase microplate immunoassay for urine survivin detection was designed based on NLuc luciferase, the bioluminescence reporter. The approach was tested on model and clinical human urine samples with the results correlating well with those obtained by a commercial colorimetric method.
Authors
- Denis V. Chernyaev (ORCID: https://orcid.org/0000-0002-4625-9531)
- Ludmila A. Frank (ORCID: https://orcid.org/0000-0003-4462-1944)
- Andrey L. Matveev (ORCID: https://orcid.org/0000-0002-6884-6104)
- Nikita S. Panamarev (ORCID: https://orcid.org/0000-0001-6954-7280)
- Р. А. Зуков (ORCID: https://orcid.org/0000-0002-7210-3020)
Institutions
- Institute of Chemical Biology and Fundamental Medicine (RU)
- Institute of Biophysics (RU)
- Krasnoyarsk Regional Clinical Hospital (RU)
- Siberian Branch of the Russian Academy of Sciences (RU)
- Siberian Federal University (RU)
Publication Details
- Journal
- Analytical Methods
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5ay00599j
- Primary Topic
- bioluminescence and chemiluminescence research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Russian Science Foundation