Novel Method for Tooth Extraction in Rabbits: Advantages and Considerations

Background: Experimental studies are essential before clinical practice in human subjects. Rabbits are mostly the first choice for basic implant design studies due to their size, easy management, short life span and economical aspects of purchase and maintenance. However, no exact protocol related to tooth extraction can be found in the open literature. Our research explores various facets of dental implants through hands-on experimental surgical investigations. Methods: A new approach to tooth extraction in adult New Zealand rabbits was evaluated. The extraction steps for the mandibular first premolar tooth under general anesthesia included a mouth opener usage, incision, mucoperiosteal flap removal, drilling, removal of bone in the mesial of the premolars, use of periodontal Gracey and surgical scraping curettes for tooth luxation, extraction of the luxated tooth, socket irrigation and primary suturing. Dental volumetric tomography images from three distinct angles: axial, coronal and sagittal of the extraction area were evaluated to check the correctness of the surgical procedure. Result: Our technique has yielded a remarkable success rate for the first premolar tooth extraction. We determined that the routine application of elevators and forceps, which are typically employed in tooth extraction procedures, was insufficient. To establish an appropriate surgical setting, we introduced a mouth opener. Our findings indicate no discernible tooth remnants and the socket appeared fully vacated. The suggested experimental model suits bone graft/implant and other dental studies.