Trichothecene Sesquiterpenes with Anti-osteosarcoma Cytotoxicity from the Fungus Fusarium sp. XPW68

Fusarinene A (1), a previously undescribed trichothecene sesquiterpene, and three known compounds, 4-hydroxytrichotheca-9,12-diene (2), trichodermol (3), and trichodermin (4), were isolated from the extract of Fusarium sp.XPW68.Their structures and relative configurations were identified by detailed analysis of nuclear magnetic resonance (NMR) and high-resolution electrospray ionization mass spectrometry (HRESIMS) data.In the cytotoxic assay, compounds 1-4 exhibited selective or potent inhibition against five human osteosarcoma cell lines including 143B, MG-63, SaOS-2, SW1353, and U2OS, with IC50 values ranging from 0.8 to 48.2 M.