Generation of healthy bovine ovarian organoids: a proof-of-concept derivation technique
This study describes an effective mechanical protocol for deriving healthy OvaOs from bovine ovaries. These 3D models faithfully replicate the biological features of bovine OSE, with sustained viability across long-term cultures, passaging, and freeze-thaw cycles. These findings underscore their potential as translational models for advancing ovarian physiology research and adapting protocols to human ovarian tissue.
Authors
- Emilio Francés‐Herrero (ORCID: https://orcid.org/0000-0002-5202-8967)
- Irene Cervelló (ORCID: https://orcid.org/0000-0002-7018-4971)
- Clara Bueno-Fernández (ORCID: https://orcid.org/0000-0003-2093-6239)
- Amparo Faus
- María Gómez‐Álvarez (ORCID: https://orcid.org/0009-0007-1862-5403)
- Marcos Agustina‐Hernández (ORCID: https://orcid.org/0000-0003-1025-8024)
- António Pellicer (ORCID: https://orcid.org/0000-0002-8254-863X)
- Paula Alonso-Frías
- Nadaya Corpas
Institutions
- Universitat de València (ES)
- Information Visualization and Innovative Research (US)
- Instituto de Investigación Sanitaria La Fe (ES)
Publication Details
- Journal
- Journal of Ovarian Research
- Published
- 2025-05-22
- DOI
- https://doi.org/10.1186/s13048-025-01673-8
- Citations
- 4
- Primary Topic
- Reproductive Biology and Fertility
- Type
- article
- Field-Weighted Citation Impact
- 5.64
Funders
- European Commission
- Generalitat Valenciana
- Instituto de Salud Carlos III