New development of Nanosilver manufacture using abelmoschus esculentus l. pods extract as Bioreductor by Tollen's method and anti-colon cancer ability

Nanosilver (AgNP) has many uses as a result of its unique properties, including cancer prevention. In a previous study, the Turkevich Method approach used to make AgNPs by utilizing polysaccharides from okra pods (ORPE) as a bioreductor produced AgNPs larger than 100 nm in size. The resulting anti-colon cancer effect causes necrosis and apoptosis when interacting with colon cancer cells. Necrosis can aggravate cancer by causing new inflammation. Tollen's method, which uses organic catalysts, has the potential to create superior AgNPs. Developing novel AgNPs to meet the standard size of nanodrugs for anti-colon cancer is the goal of this study. This research included a type of experimental research with the posttest nonequivalent control group design, which aims to find the effect of certain treatments under directed conditions of AgNP. PSA, zeta potential, SEM, TEM, X-ray spectroscopy, and UV-Vis spectrophotometry are used to test AgNP characteristics. Cell viability assays, apoptotic potential, and flow cytometry cell cycle analysis were used to evaluate AgNP's potential as an anti-colon cancer agent. The characteristics of AgNP-ORPE were observed: UV absorbance at ʎmax 198.93 nm, particle size 107.8 ± 25.47 nm with PDI of 0.87521 ± 0.001 mPas, zeta potential value -8.7 ± 0.15 mV, SEM showed AgNP in the form of asymmetric spheres, and TEM in the form of anisotropic appearing spherical asymmetrical with an average size of 50.85 nm. The viability test of living cells at a concentration of 0-30 μg/mL and evaluation of the potential for apoptosis was 79.68%. The IC50 value obtained was 21.43 μg/mL. In the cell cycle, the G0/G1 phase decreased (17%) lower than the control, and there was an accumulation in the G2/M phase of 11.0%. The new development of AgNP-ORPE production by Tollen's method produced anisotropic spherical asymmetries AgNPs with a size of 50.85 nm and good stability. It also had an anti-colon cancer effect by significantly reducing the proliferation of the colon cancer cell cycle in the G0/G1 phase and inducing apoptosis of the intrinsic pathway.