Assembly of ascovirus HvAV-3h long DNA fragment using the Transformation-Associated Recombination (TAR) approach in yeast cells
Overall, the in vivo TAR assembly method efficiently assembled a long DNA fragment derived from the ascovirus genome as a template. The process is cost-effective and can be scaled up to synthesize the entire genome for gene functional studies.
Authors
- Guo‐Hua Huang (ORCID: https://orcid.org/0000-0002-6841-0095)
- Hengrui Hu (ORCID: https://orcid.org/0000-0001-8581-7965)
- Heba A. H. Zaghloul (ORCID: https://orcid.org/0000-0002-1510-4780)
- Zhengkun Xiao (ORCID: https://orcid.org/0000-0002-8352-5755)
Institutions
- Chinese Academy of Sciences (CN)
- Wuhan Institute of Virology (CN)
- Hunan Agricultural University (CN)
- Alexandria University (EG)
Publication Details
- Journal
- BMC Biotechnology
- Published
- 2025-05-22
- DOI
- https://doi.org/10.1186/s12896-025-00964-8
- Citations
- 1
- Primary Topic
- Viral Infectious Diseases and Gene Expression in Insects
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Natural Science Foundation of China
- Chinese Academy of Sciences