Comprehensive evaluation of the MeltPro MTB/PZA assay for prediction of pyrazinamide resistance in multidrug-resistant tuberculosis

This study underscores the pressing need for reliable diagnostic methods to address high PZA resistance rates in TB cases, particularly in MDR-TB strains. By confirming that pncA mutations are the principal drivers of PZA resistance, we highlight the diagnostic potential of the MeltPro MTB/PZA assay as a rapid and effective alternative to conventional culture-based methods. Demonstrating sensitivity and specificity comparable to WGS and pDST, this assay offers a practical, accessible approach for timely PZA resistance prediction. It supports more tailored and effective MDR-TB treatment strategies, which are essential for optimizing patient care in both well-resourced and constrained settings.