EVALUATION OF PHYTOCHEMICAL ANALYSIS, METAL ION CONTENTS, IN CORRELATION TO IN VITRO ANTIOXIDANT ACTIVITY OF FICUS ELASTICA AND CYAMOPSIS TETRAGONOLOBA

The present study was aimed with the identification of potent active phytochemicals followed by HPLC determination of presence of required bioactive components in Ficus elastica and Cyamopsis tetragonoloba. Thereafter, total phenolics were estimated and compared based on phytochemical screening. Determination of metal ions from the leaves of both plants was estimated by Atomic Absorption Spectrophotometer and finally, results were correlated with the potent antioxidant activity. DPPH, and Hydrogen per oxide scavenging assay methods were carried out for the antioxidant assay.IC50 value was determined as 3.45 and 134.78 µg/ml for CT extracts in DPPH and H2O2 assays respectively. Phytochemical analysis showed presence of alkaloids, glycosides, flavonoids, phenolics, tannins, terpenoids, and sterols in Ficus elastica leaves and alkaloids, glycosides, flavonoids, phenolics, and sterols in Cyamopsis tetragonoloba beans when various chemical tests were performed. TLC was identified various alkaloids and flavonoids from both the plants. Presence of beta- sitosterol and lupeol were identified and estimated by HPLC method (3.41 and 5.37 mg beta sitosterol and lupeol respectively in CT extract and 6.48 and 3.01 mg beta sitosterol and lupeol respectively in FE extract). Metal ions viz. Fe, Mg, Cu and Zn were identified as essential heavy metals with very negligible non essential heavy metals viz. Cd, Ni, As and Pb were estimated from both the plants whereas, Na, Ca, and K were higher in both of them. Finally correlation study among the metal ions, total phenolics, with antioxidant potentiality was established and confirmed the role of plant constituents in effective said activity.