Dysregulation of miR-709 in diabetic kidney disease and its role in inflammatory response

Abstract Objectives Research has shown that miRNAs play an essential role in the pathophysiology diabetic kidney disease (DKD). This study primarily explored the expression of miR-709 in DKD and elucidated its potential clinical significance for patients with DKD. Methods A total of 95 patients with DKD were included in the case group, while 102 T2DM patients constituted the control group. 50 healthy individuals were selected as the healthy group. Real-time quantitative PCR was employed to assess the expression levels of miR-709. The receiver operating characteristic curve was used to evaluate the diagnostic value of miR-709 in DKD. Spearman correlation analysis was performed to explore the relationship between serum miR-709 levels and various clinical data variables. Furthermore, an in vitro DKD cell model was developed to investigate the effect of miR-709 expression in DKD. Results In DKD patients, serum miR-709 levels are markedly reduced and have high diagnostic value. MiR-709 associated with clinical features and inflammation in DKD. Up-regulation of miR-709 significantly suppressed HG-induced proliferation and inflammatory factor levels in human mesangial cells (HMCs). Conclusions The decreased level of miR-709 is closely linked to DKD and its inflammation, which is expected to become a potential biomarker for clinical screening and a target for therapeutic intervention.