Fabrication and characterization of hydrogel films blended with Chromolaena odorata leaf extract and their antioxidant properties

Background: Hydrogels derived from natural polymers are widely studied for biomedical applications.Durian rind, an agricultural waste, serves as a valuable cellulose source for producing Carboxymethyl Cellulose (CMC) based hydrogels.Additionally, Chromolaena odorata (C.odorata) extract enhances the antioxidant properties of the hydrogel, aiding in the reduction of oxidative stress.These properties are essential for promoting wound healing and biomedical applications.Ensuring non-cytotoxicity is crucial for safety and effectiveness, making them promising for wound dressings and drug delivery systems.Objective: To develop antioxidant-active hydrogel films from durian rind-derived CMC incorporating C.odorata extract with biocompatible and non-cytotoxic properties for potential biomedical applications. Methods:We extraced compounds from C.odorata leaves and evaluated their antioxidant, cytotoxicity, and inflammatory properties.In the synthesis of CMC from durian peel, it is utilized as a polymer to be molded into a hydrogel.The formation of hydrogel from synthetic CMC contained wih C.odorata leaf extract was optimized.Subsequently, its structure and surface were characterized by FTIR and SEM, respectively.The biocompatibility with cell lines and antioxidant activity of hydrogel were confirmed after casting.Results: This research showed that a hydrogel mixed with C.odorata leaves extract at 2X and 10X can be formed.The film was tested and demonstrated sustained antioxidant properties, and the film was non-toxic on keratinocyte, fibroblast, and macrophage cells. Conclusion:The hydrogel derived from durian rind-based CMC contained C.odorata extract, preserved its structural integrity and antioxidant activity.The films exhibited biocompatibility and non-cytotoxicity, highlighting their potential for biomedical applications like wound dressings and drug delivery systems.