Near-infrared chemiluminescent probes for monitoring leucine aminopeptidase activity
Near-infrared (NIR) chemiluminescent probes offer high sensitivity for biological sensing applications. Herein, we present two NIR chemiluminescent probes for leucine aminopeptidase (LAP) detection, designed by covalently linking the dicyanomethylene-4<i>H</i>-pyran (DCM) fluorophore to Schaap's dioxetane scaffold. The impact of substitution position on chemiluminescence kinetics and enzymatic responsiveness revealed that only the <i>para</i>-substituted probe with minimal steric hindrance enables efficient <i>in vitro</i> and <i>in vivo</i> imaging of LAP activity.
Authors
- Jing Yang (ORCID: https://orcid.org/0000-0002-3125-8691)
- Z. Y. Zhang
- Zhiqian Guo (ORCID: https://orcid.org/0000-0002-2192-825X)
- Min Liu (ORCID: https://orcid.org/0000-0002-8077-0130)
- Haoyun Xue
- Xiuyan Zhao
Institutions
- East China University of Science and Technology (CN)
- Tobacco Research Institute (CN)
- Shanghai Institute of Technology (CN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc02329g
- Citations
- 1
- Primary Topic
- Nanoplatforms for cancer theranostics
- Type
- article
- Field-Weighted Citation Impact
- 0.67
Funders
- National Natural Science Foundation of China