Analysis of Particle Size Distribution and Morphological Characterization of Transferrin-Modified Mangiferin Lipid Cubic Liquid Crystalline Nanoparticles

目的：探讨转铁蛋白(TF)修饰对芒果苷脂质立方液晶纳米粒粒径分布、晶体结构及表面形貌的优化作用，分析其在药物递送系统中的性能与应用潜力。方法：采用高效乳化法制备纳米粒，通过动态光散射(DLS)测定粒径分布，利用X射线衍射(XRD)表征晶体结构，结合扫描电子显微镜(SEM)分析表面形貌特征，考察不同TF修饰量的影响。结果：TF修饰显著优化了纳米粒的粒径分布，当TF当量为0.2时，平均粒径为98.4 nm，分布最均匀。XRD表明，TF修饰增强了立方晶系的比例和晶体稳定性。SEM显示，TF修饰提升了立方晶粒比例至86.3%，改善了表面形貌和靶向性能。结论：TF修饰有效优化了芒果苷脂质立方液晶纳米粒的粒径分布、晶体结构和表面形貌，显著提升了其靶向性和稳定性，为其在精准药物递送中的应用提供了重要数据支持。Objective: To investigate the optimization effect of transferrin (TF) modification on the particle size distribution, crystal structure and surface morphology of manciferin lipid cubic liquid crystal nanoparticles, and to analyze its performance and application potential in drug delivery systems. Methods: Nanoparticles were prepared by the efficient emulsification method. The particle size distribution was determined by dynamic light scattering (DLS). The crystal structure was characterized by X-ray diffraction (XRD). The surface morphology characteristics were analyzed in combination with scanning electron microscopy (SEM), and the influence of different TF modification amounts was investigated. Result: TF modification significantly optimized the particle size distribution of nanoparticles. When the TF equivalent was 0.2, the average particle size was 98.4 nm, with the most uniform distribution. XRD indicates that TF modification enhances the proportion and crystal stability of the cubic crystal system. SEM showed that TF modification increased the proportion of cubic grains to 86.3%, and improved the surface morphology and targeting performance. Conclusion: TF modification effectively optimizes the particle size distribution, crystal structure and surface morphology of mangiferin lipid cubic liquid crystal nanoparticles, significantly improving their targeting and stability, providing important data support for their application in precision drug delivery.