Effect of Artificial Dermis Loaded with Recombinant Human Epidermal Growth Factor on Apoptosis in Rat Wound Repair

目的：探讨负载重组人表皮生长因子的人工真皮在大鼠创面修复中对创面组织细胞凋亡的影响。方法：采用浸渍法制备负载Rh-EGF的人工真皮。随机将12只SPF级雄性SD大鼠分为两组，每组6只，适应性喂养1周后，于SD大鼠背部建立创面模型。联合组施加浸渍Rh-EGF的人工真皮，人工真皮组则应用生理盐水浸渍的人工真皮。通过TUNEL/DAPI双荧光染色法对大鼠创面组织进行细胞凋亡评估，分别对凋亡细胞数量和总细胞数量进行定量分析，最终计算得出细胞凋亡率。结果：与人工真皮组相比，试验组的细胞凋亡率显著减少(P Objective: To investigate the effect of artificial dermis loaded with recombinant human epidermal growth factor on apoptosis of traumatic tissue cells in rat wound repair. Methods: Artificial dermis loaded with Rh-EGF was prepared by impregnation method. Twelve SPF-grade male SD rats were randomly divided into two groups of 6 rats each, and the trauma model was established on the back of SD rats after 1 week of adaptive feeding. Artificial dermis impregnated with Rh-EGF was applied to the experimental group, while saline-impregnated artificial dermis was applied to the control group. Apoptosis was assessed by TUNEL/DAPI dual fluorescence staining of rat traumatic tissues, and the number of apoptotic cells and the total number of cells were quantified, respectively, and the apoptosis rate was finally calculated. Results: Compared with the control group, the apoptosis rate of the test group was significantly reduced (P 0.01). Conclusion: Artificial dermis loaded with recombinant human epidermal growth factor can reduce apoptosis during wound repair in SD rats.