Rapid visual detection of Monkeypox virus by one-step LAMP-CRISPR/Cas12b assay
In resource-limited settings, our method requires only a portable heat block or water bath and a blue light or ultraviolet flashlight for visual detection of MPXV, making it highly accessible. The integration of LAMP and CRISPR/Cas12b provides a robust, user-friendly platform for point-of-care testing, with promising potential for the rapid molecular diagnosis of infectious diseases.
Authors
- Yiyue Ge (ORCID: https://orcid.org/0000-0002-4839-5334)
- Qiao Qiao (ORCID: https://orcid.org/0000-0002-3041-8972)
- Lunbiao Cui (ORCID: https://orcid.org/0000-0001-6212-6053)
- Tao Wu (ORCID: https://orcid.org/0000-0002-9859-4534)
- Jinlei Guo (ORCID: https://orcid.org/0000-0002-4594-9696)
- Xiaojuan Zhu (ORCID: https://orcid.org/0000-0002-3782-1342)
- Ying Chi (ORCID: https://orcid.org/0000-0002-3020-7892)
- Ge Hu
- Kangchen Zhao
- Yunfeng Shan
Institutions
- Jiangsu Provincial Center for Disease Control and Prevention (CN)
Publication Details
- Journal
- Virology Journal
- Published
- 2025-05-20
- DOI
- https://doi.org/10.1186/s12985-025-02780-0
- Citations
- 5
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 4.03
Funders
- Government of Jiangsu Province