Antimicrobial Potential and HPLC Analysis of BGR-34: An Antidiabetic Ayurvedic Formulation

Background: BGR-34, a polyherbal formulation, has garnered attention for its antimicrobial properties, attributed to its diverse composition of primary and secondary me-tabolites. Objective: The objective of the study was to generate good scientific data regarding BGR -34. Methods: This study was carried out to investigate the phytochemical screening, High Per-formance Liquid Chromatography (HPLC) analysis using Quercetin as a standard, and an-timicrobial efficacy of BGR-34 against significant human pathogens, including Klebsiella pneumoniae, Vibrio cholerae, Staphylococcus epidermidis, Listeria monocytogenes, Esch-erichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella Typhi, Strepto-coccus mutans, Streptococcus pyogenes, Enterococcus faecalis, Bacillus subtilis, and Xan-thomonas oryzae. Results: The findings revealed a dose-dependent antimicrobial effect, with optimal activity observed at 100 mg/mL, highlighting its potential as a therapeutic agent. The phytochemical screening confirmed the presence of various primary and secondary metabolites, such as flavonoids, alkaloids, saponins, carbohydrates, alkaloids, tannins, proteins, terpenoids, etc. HPLC analysis demonstrated the presence of quercetin (2.2974 ug/mL), which is responsi-ble for various pharmacological activities. Antimicrobial potential showed activity of BGR-34 against various microbes that are responsible for various diseases. The bioactive com-pounds in BGR-34, such as phenolics, flavonoids, and terpenoids, exhibit mechanisms like biofilm disruption and microbial membrane interference, underscoring its broad-spectrum efficacy. Furthermore, its potential role in addressing infections related to biofilm-forming pathogens and resistant strains establishes its clinical significance. Conclusion: These results support the continued exploration of BGR-34 as a viable option in combating microbial infections, with implications for managing resistant pathogens, and reducing the burden of infectious diseases.