Enhancing the Denitrification Performance of <i>Rhodococcus</i> <i>biphenylivorans</i> XYH-1 through Bamboo Biochar

本研究通过实验探讨竹炭在不同环境条件下对菌株Rhodococcus biphenylivorans XYH-1的强化效果，分别考察了竹炭投加量、细菌接种量、碳氮比、初始pH、温度等因素对硝酸盐降解效率的影响，得出了以下结论：竹炭-XYH-1复合体系降解硝酸盐的最优条件是：竹炭投加量15 g，细菌接种量1%，碳氮比20:1，初始pH = 7，温度35℃。在此最佳条件下，竹炭-XYH-1复合体系降解硝酸盐的效率达到了80.5%。竹炭能够强化菌株XYH-1反硝化性能。将竹炭应用于硝酸盐降解体系，结果表明，负载竹炭的菌株XYH-1对硝酸盐的降解率达到80.5%，而未负载竹炭的菌株XYH-1对硝酸盐的降解率仅为64.7%，负载竹炭后菌株对硝酸盐的降解率可提高15.8%，说明竹炭可以强化菌株XYH-1的硝酸盐降解能力。This study experimentally investigated the enhancing effects of bamboo biochar on the denitrification performance of Rhodococcus biphenylivorans XYH-1 under varying environmental conditions. Key factors including bamboo biochar dosage, bacterial inoculation amount, C/N ratio, initial pH, and temperature were systematically evaluated for their impacts on nitrate degradation efficiency. The following conclusions were drawn: The optimal conditions for nitrate degradation in the bamboo biochar-XYH-1 composite system were determined as follows: bamboo biochar dosage of 15 g, bacterial inoculation amount of 1%, C/N ratio of 20:1, initial pH of 7, and temperature of 35˚C. Under these optimized conditions, the nitrate degradation efficiency reached 80.5%. Bamboo biochar significantly enhanced the denitrification capability of strain XYH-1. Experimental results demonstrated that the nitrate degradation rate of bamboo biochar-loaded XYH-1 reached 80.5%, compared to 64.7% for the non-biochar group, indicating a 15.8% improvement. This confirms the critical role of bamboo biochar in boosting the nitrate removal capacity of strain XYH-1.