Comparative evaluation of sequencing technologies and primer sets for mouse gut microbiota profiling
These results highlight the advantages and limitations of different sequencing strategies in microbiota research. While 16S rRNA sequencing remains a cost-effective tool for assessing bacterial diversity, MS provides superior taxonomic resolution and more precise species identification. Our study advocates for a hybrid approach that combines multiple sequencing technologies to achieve a more comprehensive and accurate representation of microbial communities.
Authors
- Daria I. Boldyreva (ORCID: https://orcid.org/0000-0003-2573-7634)
- Vladimir A. Veselovsky (ORCID: https://orcid.org/0000-0002-4336-9452)
- Andrey K. Larin (ORCID: https://orcid.org/0000-0002-9429-792X)
- Evgenii I. Olekhnovich (ORCID: https://orcid.org/0000-0003-4899-342X)
- Maya V. Odorskaya (ORCID: https://orcid.org/0000-0002-9821-9865)
- Vladislav V. Babenko (ORCID: https://orcid.org/0000-0003-2242-2127)
- В. Н. Даниленко (ORCID: https://orcid.org/0000-0001-5780-0621)
- Anna V. Kudryavtseva (ORCID: https://orcid.org/0000-0002-3722-8207)
- Oksana V. Selezneva
- Ksenia M. Klimina (ORCID: https://orcid.org/0000-0002-5563-644X)
- N.V. Zakharevich
- Roman А. Yunes (ORCID: https://orcid.org/0000-0001-6283-6258)
- Vladislav Pavlov (ORCID: https://orcid.org/0000-0002-2942-6393)
- Maxim D. Morozov (ORCID: https://orcid.org/0000-0001-6128-0921)
- Polina Y. Zoruk
- S. D. Koldman
- V. A. Koldman
- Aleksandra Strokach
Institutions
- Engelhardt Institute of Molecular Biology (RU)
- Vavilov Institute of General Genetics (RU)
- Federal Medical-Biological Agency (RU)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2025-05-20
- DOI
- https://doi.org/10.3389/fmicb.2025.1584359
- Citations
- 6
- Primary Topic
- Gut microbiota and health
- Type
- article
- Field-Weighted Citation Impact
- 4.84
Funders
- Russian Science Foundation