pTripleTREP – A vector for tightly controlled expression and purification of virulence factors in Staphylococcus aureus
The expression vector pTripleTREP adds an important element to the staphylococcal molecular toolbox, facilitating the tightly controlled homologous expression and rapid native purification of secreted staphylococcal virulence factors. The optimised architecture and genetic features of the vector additionally provide a solid background for further applications such as plasmid-based complementation or interaction studies. Thus, pTripleTREP will support research on the role of staphylococcal virulence factors, paving the way for future therapeutic strategies to combat this pathogen.
Authors
- Janina Lange
- Hannes Wolfgramm (ORCID: https://orcid.org/0000-0002-6028-0046)
- Leif Steil (ORCID: https://orcid.org/0000-0002-0733-8421)
- Christian Hentschker
- Manfred Schedlowski (ORCID: https://orcid.org/0000-0002-9968-9770)
- Manuela Gesell Salazar (ORCID: https://orcid.org/0000-0002-6727-1978)
- Kristin Surmann (ORCID: https://orcid.org/0009-0009-7305-3457)
- Stephan Michalik (ORCID: https://orcid.org/0000-0002-6550-0132)
- Uwe Völker (ORCID: https://orcid.org/0000-0002-5689-3448)
- Alexander Reder (ORCID: https://orcid.org/0009-0005-3575-6849)
- Larissa Milena Busch (ORCID: https://orcid.org/0000-0002-9545-6355)
- Marco Harms
- Christopher Saade
Institutions
- Universitätsmedizin Greifswald (DE)
- Universität Greifswald (DE)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-05-20
- DOI
- https://doi.org/10.1186/s12934-025-02736-7
- Citations
- 6
- Primary Topic
- Antimicrobial Resistance in Staphylococcus
- Type
- article
- Field-Weighted Citation Impact
- 8.38