THE EFFICACY OF SERUM PEPSIN IN MUSCLE TISSUE PEPTOLYSIS

The veterinary-sanitary examination occupies a leading place in the system of antitrichinellosis measures and protection of population and animals from trichinellosis. In examining large volumes of carcasses and meat products, as well as the need to reduce economic costs in the examination for trichinellosis, the most promising method is automated muscle tissue peptolysis using AVT (Gastros) type devices. One of the main components of the artificial digestion technology of muscle samples is pepsin with an optimal enzyme content, which allows for effective peptolysis of muscle tissue, and high-quality examination for trichinellosis, accordingly. A definite disadvantage when using pepsin is limited duration of its use due to decreased enzyme activity. To do this, its periodic testing is necessary. The purpose of this research was to determine the digestive capacity of domestic serum pepsin produced by Shako LLC as a base preparation for veterinary and sanitary examination laboratories, scientific and production specialized structures and OPVC of meat-processing factories. Based on the data obtained, the prospects for the widespread use of pepsin produced by Shako LLC have been determined for these purposes in accordance with the Methodical Guidelines for laboratory diagnosis of animal trichinellosis.