Discovery and Genome Characterization of Three New Rhabdoviruses Infecting Passiflora spp. in Brazil

This study aimed to explore the RNA viruses affecting <i>Passiflora</i> species in Brazil. Our results enhance the understanding of the viruses that infect <i>Passiflora</i> plants by identifying and characterizing three previously unrecognized viruses: Passiflora cytorhabdovirus (PFCV), Passiflora nucleorhabdovirus 1 (PaNV1), and Passiflora nucleorhabdovirus 2 (PaNV2). These rhabdoviruses were identified through high-throughput sequencing and validated by reverse transcription-polymerase chain reaction (RT-PCR) in various <i>Passiflora</i> species. PFCV has a genome organization 3'-N-P-P3-P4-M-G-P7-L-5' and was classified as a novel member of the <i>Gammacytorhabdovirus</i> genus. A particularly noteworthy feature of PFCV is its glycoprotein, as the genomes of other gammarhabdoviruses do not contain this gene. PFCV has a high incidence across multiple locations and was identified in plants from Northeastern, Central, and Southeastern Brazil. PaNV1 with genome structure 3'-N-P-P3-M-G-L-5' and PaNV2 with genome organization 3'-N-X-P-Y-M-G-L-5' are new members of the <i>Alphanucleorhabdovirus</i> genus and have a more restricted occurrence. Importantly, all three viruses were found in mixed infections alongside at least one other virus. In situ observations confirmed mixed infections, with PaNV2 particles co-located in tissues with a potyvirus and a carlavirus. Phylogenetic and glycoprotein sequence similarity network analysis provided insights into their evolutionary placement and potential vector associations. These findings expand the known diversity of rhabdoviruses in <i>Passiflora</i> and contribute to the understanding of their evolution and epidemiology.

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Journal
Viruses
Published
2025-05-19
DOI
https://doi.org/10.3390/v17050725
Citations
1
Primary Topic
Plant Virus Research Studies
Type
article
Field-Weighted Citation Impact
1.75

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article

Discovery and Genome Characterization of Three New Rhabdoviruses Infecting Passiflora spp. in Brazil

Rosana Blawid, Rafaela S. Fontenele, Andreza Henrique Vidal, Elliot Watanabe Kitajima et al.
1 citations
Viruses
Plant Virus Research Studies
1.75
article

Discovery and Genome Characterization of Three New Rhabdoviruses Infecting Passiflora spp. in Brazil

Rosana Blawid, Rafaela S. Fontenele, Andreza Henrique Vidal, Elliot Watanabe Kitajima, Yam Sousa Santos, Onildo Nunes de Jesus, Fernando Lucas de Melo, Arvind Varsani, José Leonardo Santos-Jiménez, Bruna Pinheiro-Lima, Cristiano Lacorte, Márcio Martinello Sanches, Simone G. Ribeiro, Ana Clara Rodrigues Abreu, Magnólia de Araújo Campos, Maité F. S. Vaslin, D. M. T. Alves-Freitas, Gustavo Pereira Felix, Fábio Gelape Faleiro, Monique Jacob Xavier Vianna, E. F. M. Abreu, Raul Castro Carrielo Rosa, Isadora Nogueira, Jorge Flávio Sousa Dantas-Filho
article en
1 citations

Abstract

This study aimed to explore the RNA viruses affecting <i>Passiflora</i> species in Brazil. Our results enhance the understanding of the viruses that infect <i>Passiflora</i> plants by identifying and characterizing three previously unrecognized viruses: Passiflora cytorhabdovirus (PFCV), Passiflora nucleorhabdovirus 1 (PaNV1), and Passiflora nucleorhabdovirus 2 (PaNV2). These rhabdoviruses were identified through high-throughput sequencing and validated by reverse transcription-polymerase chain reaction (RT-PCR) in various <i>Passiflora</i> species. PFCV has a genome organization 3'-N-P-P3-P4-M-G-P7-L-5' and was classified as a novel member of the <i>Gammacytorhabdovirus</i> genus. A particularly noteworthy feature of PFCV is its glycoprotein, as the genomes of other gammarhabdoviruses do not contain this gene. PFCV has a high incidence across multiple locations and was identified in plants from Northeastern, Central, and Southeastern Brazil. PaNV1 with genome structure 3'-N-P-P3-M-G-L-5' and PaNV2 with genome organization 3'-N-X-P-Y-M-G-L-5' are new members of the <i>Alphanucleorhabdovirus</i> genus and have a more restricted occurrence. Importantly, all three viruses were found in mixed infections alongside at least one other virus. In situ observations confirmed mixed infections, with PaNV2 particles co-located in tissues with a potyvirus and a carlavirus. Phylogenetic and glycoprotein sequence similarity network analysis provided insights into their evolutionary placement and potential vector associations. These findings expand the known diversity of rhabdoviruses in <i>Passiflora</i> and contribute to the understanding of their evolution and epidemiology.

VirusesVol. 17(5)
Universidade Federal do Rio de Janeiro (BR), Universidade Federal de Viçosa (BR), Universidade de Brasília (BR), Brazilian Agricultural Research Corporation (BR), Universidade Federal de Campina Grande (BR), Secretaria de Agricultura e Abastecimento (BR), Genomic (Brazil) (BR), Arizona State University (US), Universidade Federal Rural de Pernambuco (BR)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundação de Apoio à Pesquisa do Distrito Federal, Embrapa Mandioca e Fruticultura
Openalex Percentile: Top 15%
Plant Virus Research Studies
1.75
DOIOpen Access PDFOpenAlex
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