SELECTION OF ADJUVANTS IN THE DESIGN OF A VACCINE AGAINST VIRAL RESPIRATORY INFECTIONS AND PASTEURELLOSES IN CALVES

The purpose of the study is to select optimal adjuvants in the design of a polyvalent vaccine against infectious rhinotracheitis, viral diarrhea, parainfluenza-3, respiratory syncytial infection and bovine pasteurellosis. The vaccine was prepared under the conditions of BelVitunifarm OJSC. The vaccine contains avirulent formaldehyde-inactivated strains of infectious rhinotracheitis viruses, diarrhea, parainfluenza-3, M. haemolytica and P. multocida (type A and B), recombinant strain of E. coli with genome of PC-virus, oily adjuvant. Viruses were produced separately in MDBK cell culture, where they accumulated in the following titers: infectious rhinotracheitis – 6.5 lg TCD 50/ml, diarrhea virus – 7.0 lg TCD 50/ml, parainfluenza-3 virus – 5.5 lg TCD 50/ml. Accumulation of M. haemolytica and P. Multocida was carried out on Hottinger broth to a concentration of – 3.0 billion microbial bodies in 1 ml, a recombinant strain of Escherichia coli with the PC virus genome on a nutrient medium containing peptone, yeast extract, glycerol, phosphates, chlorides and sulfates to a concentration of – 3.0 billion microbial bodies in 1 ml. Studies on the selection of an adjuvant in the Pastevir-R vaccine against infectious rhinotracheitis, viral diarrhea, parainfluenza-3, respiratory syncytial infection and pasteurellosis were carried out in OAO “Palminki”, Gorodok district, Vitebsk region, on calves. It was found that in the development of a vaccine against viral-bacterial infections of young cattle, higher immunogenicity indicators reflecting the stimulation of the humoral immune response were obtained using Montanida IZA 201 oil depositing substances (Montanidae, Seppic, France) at a concentration of 50%.