IMMUNOHISTOCHEMICAL CHARACTERISTICS OF PANCREATIC ENDOCRINECYTES IN THE RACCOON DOG

The raccoon dog is a typical representative of the predators of the Polesie State Radiation and Ecological Reserve. Like other predators, it can serve as a bioindicator of the state of the natural environment, so the study of its pancreas at the histological level using the immunohistochemical method is of great interest for scientific research. The immunohistochemical method helped determine the nature of the cells present in the islets of Langerhans in the pancreas of the raccoon dog. Different types of distribution of endocrine cells containing insulin and glucagon were detected using double staining with antibodies to insulin and glucagon in the pancreas of the raccoon dog. Antibodies to insulin were used to identify B cells, and antibodies to glucagon – A cells. A cells in the islets of Langerhans showed strong immune reactivity and were brown-golden in color when incubated with glucagon antibodies, with immunoreactive cells occupying most of the islets.