Single nanoparticle analysis-based CRISPR/Cas12 bioassay for amplification-free HIV detection
To reduce the "window period" in HIV detection, most analytical methods require additional enzymes for signal amplification. Exempting challenges like primer interference and false positives in amplification strategies, we developed an amplification-free bioassay that uses CRISPR's potent cleavage activity and the competent sensitivity of single-nanoparticle analysis. An attomolar detection limit was achieved with adequate selectivity. Serum and cell tests confirm the bioassay's accurate and sensitive HIV detection.
Authors
- Chengchao Zhang (ORCID: https://orcid.org/0009-0000-5852-493X)
- Yi Lv (ORCID: https://orcid.org/0000-0002-7104-2414)
- Lin Xu (ORCID: https://orcid.org/0000-0001-5815-4457)
- Zili Huang (ORCID: https://orcid.org/0000-0001-7312-5996)
- Rui Liu (ORCID: https://orcid.org/0000-0001-9928-5373)
- Lichun Zhang (ORCID: https://orcid.org/0000-0002-4441-2176)
- Xiao Chen (ORCID: https://orcid.org/0000-0002-7634-9319)
- Yanlin Chen (ORCID: https://orcid.org/0000-0003-1016-3535)
Institutions
- National University of Singapore (SG)
- Sichuan University (CN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc01716e
- Citations
- 3
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 2.42
Funders
- National Natural Science Foundation of China
- Sichuan Province Science and Technology Support Program
- Fundamental Research Funds for the Central Universities
- Outstanding Youth Science and Technology Talents Program of Sichuan