Cirsium setosum Extract-Loaded Hybrid Nanostructured Scaffolds Incorporating a Temperature-Sensitive Polymer for Mechanically Assisted Wound Healing

<b>Background/Objectives</b>: <i>Cirsium setosum</i> (commonly known as thistle) is a traditional Chinese medicinal plant with significant therapeutic potential, exhibiting hemostatic, antioxidant, and wound-healing properties. Electrospinning offers a versatile platform for fabricating nanoscale scaffolds with tunable functionality, making them ideal for drug delivery and tissue engineering. <b>Methods</b>: In this study, a bioactive extract from thistle was obtained and incorporated into a thermosensitive triblock copolymer (PNNS) and polycaprolactone (PCL) to develop a multifunctional nanofibrous scaffold for enhanced wound healing. The prepared nanofibers were thoroughly characterized using Fourier-transform infrared spectroscopy (FTIR), contact angle measurements, thermogravimetric analysis (TGA), and tensile fracture testing to assess their physicochemical properties. <b>Results</b>: Notably, the inclusion of PNNS imparted temperature-responsive behavior to the scaffold, enabling controlled deformation in response to thermal stimuli-a feature that may facilitate wound contraction and improve scar remodeling. Specifically, the scaffold demonstrated rapid shrinkage at a physiological temperature (38 °C) within minutes while maintaining structural integrity at ambient conditions (20 °C). In vitro studies confirmed the thistle extract's potent antioxidant activity, while in vivo experiments revealed their effective hemostatic performance in a liver bleeding model when delivered via the composite nanofibers. Thistle extract and skin temperature-responsive contraction reduced the inflammatory outbreak at the wound site and promoted collagen deposition, resulting in an ideal wound-healing rate of above 95% within 14 days. <b>Conclusions</b>: The integrated strategy that combines mechanical signals, natural extracts, and electrospinning nanotechnology offers a feasible design approach and significant technological advantages with enhanced therapeutic efficacy.