Exopolysaccharide of goat milk kefir as an anti-obesity agent: Inhibition of adipogenesis enzyme activity on 3T3-L1 adipocyte model cells

Obesity is increasingly a leading cause of dyslipidemia and metabolic syndrome, which are known to be comorbidities of various diseases. A study was conducted to evaluate the effects of different concentrations of exopolysaccharide from goat milk kefir (EPS-GMK) on the activity of key enzymes involved in fat cell formation in 3T3-L1 adipocyte cells. The study used six groups, including two control groups (negative and positive) and four treatment groups with varying concentrations of EPS-GMK (25, 50, 75, and 100 µg/ml). The results showed that EPS-GMK has a significant inhibitory effect on the activity of key enzymes involved in fat cell formation. The findings suggest that EPS-GMK could be a valuable component in developing anti-obesity functional foods. This study aims to analyze the effects of EPS-GMK on the 3T3-L1 adipocyte cells' phosphoenolpyruvate carboxykinase, Glucose-6-Phosphate Dehydrogenase, and Lipoprotein lipase administration. This study applied different doses of EPS-GMK to 3T3-L1 adipocytes with four replications. The doses used were P1 (25 g/ml), P2 (50 g/ml), P3 (75 g/ml), and P4 (100 g/ml). The study also included two control groups, negative control and positive control. This study utilized a completely randomized design for maximum efficacy. The experimental material was 3T3-L1 adipocyte cells, each sample containing adipocyte model cells. One hundred six cells per well were induced with DMI on 24 culture plates. Statistical variance analysis was used to determine the significance between the mean values. The study results revealed that EPS-GMK concentrations between 25 and 100 g/mL could potentially reduce adipogenesis enzyme activity. According to this study, EPS-GMK has the potential and feasibility of being used as an anti-obesity agent.